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- Publication date: Available online 18 May 2025Source: Journal of ProteomicsAuthor(s): Vasti T. Juárez-González, Eduardo Luján-Soto, Paola Aguirre de la Cruz, Mayra Aquino-Luna, Jorge Herrera-Díaz, Brenda A. López-Ruiz, Tzvetanka D. Dinkova
- Publication date: Available online 17 May 2025Source: Journal of ProteomicsAuthor(s): Brittannie Willis, Harry J. Whitwell
- Publication date: Available online 17 May 2025Source: Journal of ProteomicsAuthor(s): Eliel Ruiz-May, Esaú Bojórquez-Velázquez, Humberto Carrasco-Vargas, Silverio Soto-Alvarez, Laura S. Rangel-Cova, Marco Antonio Vargas-Hernández, José M. Elizalde-Contreras, Víctor M. Torres De La Cruz, Armando Vilchis-Ordoñez, Miguel Ángel Palomo-Colli, Tania Angeles Floriano, Israel Parra Ortega, Rosana Pelayo-Camacho, Lidia F.E. Huerta-Nuñez
- ABSTRACT Inocutis tamaricis is a medicinal fungus with significant pharmacological activity. In this study, ultra-high performance liquid chromatography-high resolution mass spectrometry (UPLC/MS) was used to conduct a comprehensive non-targeted metabolomics analysis of the metabolites of wild fruiting body (WF), cultured fruiting body (CF), and liquid cultured mycelium (LCM). Orthogonal partial least square discriminant analysis (OPLS-DA) was used to compare the three culture methods, to describe the regulatory effect of specific culture methods on metabolite distribution and yield of I. tamaricis, […]
- Biomedical Chromatography, Volume 39, Issue 7, July 2025.
- Orbitrap (OT) -based mass spectrometer platforms are a gold standard in high-resolution mass spectrometry, where their primary disadvantage is slower-scanning speed in comparison to time-of-flight or linear ion trap mass analyzers. In this study, we utilize long OT transients to extend the precursor dynamic range by modifying the selected ion monitoring method to multiplex several precursor m/z ranges from 400 to 1000 m/z into a single scan called "Multiple Accumulation Precursor Mass Spectrometry" (MAP-MS). Our approach requires no software or […]
- Publication date: Available online 17 May 2025Source: TrAC Trends in Analytical ChemistryAuthor(s): Fangfang Wang, Lihua Zuo, Mengyuan Lv, Yuyang Wang, Ruobing Ren, Di Chen, Zhi Sun
- Publication date: Available online 16 May 2025Source: TrAC Trends in Analytical ChemistryAuthor(s): Jonathan MAURER, Helena VANLUCHENE, Athanasios TSALMPOURIS, Kris MORREEL, Julien CAMPERI, Koen SANDRA, Davy GUILLARME
- Journal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.5c00071
- Publication date: Available online 16 May 2025Source: Journal of Chromatography AAuthor(s): Veronika Pilařová, Kateřina Plachká, Taťána Gazárková, Frantisek Svec, Jean-Christophe Garrigues, Lucie Nováková
- Analytical ChemistryDOI: 10.1021/acs.analchem.5c00267
- Protein-level cellular dynamics, including multimerization, play a crucial role in rapid adaptation of plants during developmental transitions and environmental stresses. The gaseous hormone ethylene is a key regulator of these rapid cellular adjustments, especially in growth inhibition. During soil emergence, etiolated seedlings undergo crucial morphological changes to their apical hooks and hypocotyls, with ethylene inhibiting hypocotyl axial elongation while promoting radial expansion. Ethylene triggers these growth responses within two hours; however, the protein machineries and cellular processes that mediate morphogenesis […]
- Wow. Okay, so I was a little (lot) less excited about the desciption of this technology when sea slug neurons were being profiled when the technology was first described here. Those sea slug neurons can be really convenient to work with since they can grow out on a plate to be easily 100x larger than typical cells. However – these are rat brain cells and these are going to be biologically relevant to more than just sea slug biology! They start with […]
- European Journal of Mass Spectrometry, Ahead of Print. The nominal mass of amino acid residues calculated from their elemental compositions are defined by prime numbers far more often than chance, and such residues appear to play an important role in the formation and biology of proteins. It is proposed …
- J. Anal. At. Spectrom., 2025, Accepted ManuscriptDOI: 10.1039/D5JA00079C, Paper Open Access   This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.Gustavo Rossato Bitencourt, Paola de Azevedo Mello, Patricia Grinberg, Ralph Edward SturgeonSimultaneous determination of Cl, Br and I by aerosol-assisted photochemical vapor generation (PVG) with detection by inductively coupled plasma mass spectrometry (ICP-MS) was investigated. The photoreactor comprised a modified cyclonic…The content of this RSS Feed (c) The Royal Society of Chemistry
- All cells respond to changes in both their internal milieu and the environment around them through the regulation of their genes. Despite decades of effort, there remain huge gaps in our knowledge of both the function of many genes (the so-called y-ome) and how they adapt to changing environments via regulation. Here we describe a joint experimental and theoretical dissection of the regulation of a broad array of over 100 biologically interesting genes in E. coli across 39 diverse environments, […]
- The SOS response is among the most conserved pathways that promote the acquisition of antibiotic resistance in bacteria. This study aimed to identify and characterize small-molecule inhibitors of the SOS response system in the opportunistic pathogen Pseudomonas aeruginosa. A library of 318 drug-like compounds was screened for inhibition of RecA-induced LexA autoproteolysis, a key step in SOS activation. One hit compound, 3-(2-sulfanylanilino)propanoic acid, showed dose-dependent inhibition with an IC50 in the mid-micromolar range. Differential scanning fluorimetry and isothermal titration calorimetry […]
- Mass spectrometry-based immunopeptidomics is a powerful approach to uncover peptides presented by human leukocyte antigen (HLA) molecules that can guide vaccine design and immunotherapies. While data-dependent acquisition (DDA) has been the standard for navigating through the complexity associated with non-enzymatic immunopeptide database searches, data-independent acquisition (DIA) is increasingly adopted in immunopeptidomics research. In this work, we compare diaPASEF to conventional ddaPASEF in terms of global immunopeptidome profiling and bacterial epitope discovery of the model intracellular pathogen Listeria monocytogenes. We show […]
- Analytical ChemistryDOI: 10.1021/acs.analchem.5c00757
- The transmembrane protein CMTM6 was shown to promote plasma membrane expression of immune checkpoint PD-L1, an important suppressor of anti-tumor immunity. Targeting of CMTM6 was proposed as a strategy to decrease surface PD-L1 and trigger cytotoxicity against tumors. In accord, ablation of CMTM6 in mouse cancer models was shown to efficiently suppress tumor growth in a manner partially independent of PD-L1, which suggested that CMTM6 might regulate other proteins involved in anti-tumor immunity. Using mass spectrometry, we discovered that mouse […]
- RNA modifications, especially m6A in human mRNA, are believed to be dynamically regulated through RNA writers and erasers. The key eraser of m6A is ALKBH5 with its function well proven in vitro, while in vivo evidence is lacking. Here, we set out to exploit nucleic acid isotope labelling coupled mass spectrometry (NAIL-MS) in a pulse chase set-up to study the in vivo function of ALKBH5 on human RNAs. For this we purified poly(A) from whole cell total RNA and found […]
- Argonaute proteins are essential players in RNA silencing pathways, and their N-terminal extensions, particularly in the PIWI clade, often harbor conserved sequences like RG motifs. Despite their prevalence in Argonaute proteins, the role of these motifs remains poorly understood. In this study, we focus on the RG motifs within the N-terminal region of Caenorhabditis elegans PRG-1, a PIWI clade Argonaute. Using sequence alignment across Caenorhabditis species, we identify three conserved RG motifs that are methylated, as confirmed by mass spectrometry. […]
- Journal of Agricultural and Food ChemistryDOI: 10.1021/acs.jafc.5c01731
- Publication date: Available online 14 May 2025Source: Journal of Chromatography BAuthor(s): Hanna F. Roenfanz, David P. Nicolau, Joseph L. Kuti
- Publication date: Available online 14 May 2025Source: International Journal of Mass SpectrometryAuthor(s): Aleksandra Antevska, Gaoyuan Lu, Emmanuel Nkyaagye, Sarah S. Hirschbeck, Connor C. Long, Lingjun Li, Thanh D. Do